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KMID : 1007520040130010021
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Food Science and Biotechnology
2004 Volume.13 No. 1 p.21 ~ p.25
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Enzymatic Synthesis of Isoamyl Ester in Aqueous Media Optimization Using a Central Composite Design
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Gabriela A. Macedo
Glaucia M. Pastore
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Abstract
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Catalytic action of two non-commercial lipases (E.C. 3.1.1.3) during isoamyl acetate synthesis in a solvent-free system was tested using experimental design to better understand relationship between reaction variables. Yields ranging from 67 to 77% molar conversion were achieved. Synthesis of isoamyl acetate catalyzed by the chosen lipase (Rhizopus sp.) was optimized, to maximize the molar conversion (yield, Y) in a solvent-free system. Response surface methodology involving substrate molar ratio (RM) and amount of lipase (L) was used, and a second-order model was employed to generate response surfaces. Optimum conditions to obtain Y max ＝ 80% were：RM＝(2：1), L＝8.7% (w/w), 40℃, and 48hr of reaction time.
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KEYWORD
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